Base de dados : HANSEN Pesquisa : TRIFOSFATO DE ADENOSINA/IMUNOL [Descritor de assunto] Referências encontradas : 3 [refinar] Mostrando: 1 .. 3   no formato [Detalhado]

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Id: 27100 Autor: Katoch, Vishwa M; Katoch, Kiran; Ramanathan, Usha; Sharma, Vishnu D; Shivannavar, Chinappa T; Datta, Arun K; Bharadwaj, Ved P. Título: Effect of chemotherapy on viability of Mycobacterium leprae as determined by ATP Content, morphological index and FDA-EB fluorescent staining. Fonte: Int J Lep;57(3):615-620, sept. 1989. ^btab. Resumo: Viable bacterial populations were estimated in bacilli purified from 105 biopsies from 40 untreated and 65 multibacillary leprosy patients treated with multidrug therapy (MDT) for varying periods. The bacilli were purified and viability was determined by ATP content, morphological index (MI), and fluorescein diacetate-ethidium bromide (FDA-EB) staining. Viable populations were calculated, taking 3.58 x 10(-15) g/solid bacillus as the mean ATP content of a viable unit of Mycobacterium leprae. The proportion of viable bacilli was also estimated in the same specimens using solid-staining (MI) and green-staining bacilli by the FDA-EB method. In the untreated cases, the positive viability by ATP assay was 100%, 92% by MI, and 100% by FDA-EB. ATP content per solid bacillus was relatively constant, which was not the case with ATP content per green-staining bacillus. While the MI was zero in all cases, viable bacilli could still be detected by ATP estimations in 5 of the 32 (16%) patients after 2 years of MDT and in 1 of the 20 (5%) patients after 3 years of MDT. No viable bacilli could be detected even by this method beyond 3 years of MDT. On the other hand, green-staining bacilli were demonstrable in 7/32 (22%) of cases after 2 years of MDT, 2/20 (10%) after 3 years of MDT, and 1/13 (8%) after more than 3 years of treatment, indicating that the FDA-EB staining and ATP assay did not detect the same populations. A determination of the ATP content of M. leprae could be used as a reliable and sensitive tool for determining viability of the bacilli^ien. Descritores: Mycobacterium leprae/genet Mycobacterium leprae/imunol Trifosfato de Adenosina/imunol Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n3/v57n3a03.pdf - en. Localização: Br191.1

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Id: 26770 Autor: Nakamura, Masahiro; Matsuoka, Masanori. Título: Limited ATP generation in cells of mycobacterium leprae thai-53 strain in enriched kirchner liquid medium containing adenosine. Fonte: Int. J. Lepr;69(1):13-20, Mar., 2001. ilus, tab, graf. Resumo: The ATP generation in cells of Mycobacterium leprae Thai-53 strain takes place in vitro when the cells are cultivated in Kirchner liquid medium, pH 7.0, enriched with egg-yolk solution, pyruvate, transferrin, and adenosine at 30 degrees C. Among the supplements, adenosine was key and critical for the ATP generation. The optimal concentration of adenosine was 50 micrograms/ml of the medium. ATP generation, however, was limited; the rates of increase in ATP content extracted from the cells were approximately two- to threefold compared to that of the starting samples, and the increase reached a maximum at 4 or 6 weeks after incubation. No significant ATP generation in M. leprae cells was demonstrated in medium at pH 6.2 or pH 6.6, in the original Kirchner medium with or without adenosine, or when cultured at 37 degrees C, or when containing an antileprosy drug. No detectable increase in the number of M. leprae cells was observed with the increase in intracellular ATP content and DNA replication. No effect was seen with renewal of the cultured medium by freshly prepared medium at 6 weeks' cultivation on the progressive ATP generation in M. leprae. (AU)^ien. Descritores: Trifosfato de Adenosina/imunol Trifosfato de Adenosina/fisiol Adenosina/imunol Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1a02.pdf - en. Localização: BR191.1

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Id: 26719 Autor: Gupta, U. D; Katoch, K; Sharma, R. K; Singh, H. B; Natragan, M; Singh, D; Sharma, V. D; Chauhan, D. S; Das, Ram; Srivastava, K; Katoch, V. M. Título: Analysis of quantitative relationship between viability determination in leprosy by MFP, ATP bioluminescence and gene amplification assay. Fonte: Int. J. Lepr;69(4):328-334, Dec., 2001. tab. Resumo: Two hundred twenty-one untreated, borderline lepromatous/lepromatous (BL/LL) leprosy patients have been investigated for viability by the mouse foot pad method (MFP), adenosine triphosphate (ATP) and polymerase chain reaction (PCR). The biopsies were collected at the beginning of and 12/24 months after treatment. The patient group was treated with a) immunotherapy (BCG/Mw) + MDT; b) MDT + pyrazinamide; c) control MDT; d) MDT + minocycline 100 mg once a month supervised + ofloxacin 400 mg once a month supervised. Biopsies were divided in three parts for use in the mouse foot pad, molecular and ATP investigations. In untreated and treated patients (at 12 and 24 months), there was a general agreement among all three techniques, and PCR and ATP showed higher positivity as compared to MFP. Further, there was good correlation among the viable biomass estimated by bacillary ATP levels, PCR assay and growth in mouse foot pads. The positivity was observed by MFP as well as PCR assay (18-kDa and 36-kDa) from all of the specimens when the ATP content was more than 3.6 pg/million. When the ATP content was below 3.5 pg/million, the positive takes in MFP decreased but the PCR positivity correlated with ATP bioluminescence up to 0.04 pg/million. When the ATP content was even lower, the uptake in the MFP was possibly a matter of chance, while PCR positivity was observed in 96% of the cases. For specimens with undetectable ATP, positivity was seen in 1% of the cases, showing the inability of ATP bioluminescence method to detect low background due to host ATP. PCR signals in some cases could be due to the higher sensitivity of the method or persistence of DNA after bacterial death in some cases. On the whole, the PCR methods even though targeting DNA have shown good correlations with biomass which confirm their usefulness in monitoring therapeutic responses in leprosy. (AU)^ien. Descritores: Análise Quantitativa/métodos Trifosfato de Adenosina/uso diag Trifosfato de Adenosina/imunol Proteínas Luminescentes/uso diag Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n4/v69n4a04.pdf - en. Localização: BR191.1

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